Msds restore plus western strip buffer
Tired of re-running electrophoresis gels and waiting to see your results? Although optimizing assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Optimization Assay Conditions Using Pierce SuperSignal West Substrates, Figure 1 shows how the secondary antibody concentrations are optimized after a single stripping and re-probing cycle. A chemiluminescent detection system is the most sensitive method to detect any reagent still bound after the stripping procedure.
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Restore™ PLUS Western Blot Stripping Buffer
Pierce Biotechnology - Restore and Restore PLUS Western Blot Stripping Buffer
Don't have a profile? Product Code. No offer available. View All Antibodies. Antibodies Advanced Search. Biochemicals and Reagents.
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Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Don't have a profile? Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. View more versions of this product. Catalog No. Highlights: Ready and easy to use — no dilution necessary; no offensive odors; store at room temperature Compatible — use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate Cost effective — save valuable time and samples; strip blots effectively the first time Robust yet gentle — transferred proteins remain viable; strip the same blot up to five times Flexible — strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies This product s resides on a Fisher Scientific GSA or VA contract.
Western blot stripping and reprobing performance of three stripping buffers on PVDF. PA at 0. MB 15 minutes at room temperature s.
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